Protein Structure
We use X-ray crystallography, cryo-EM, and NMR to determine protein structures, characterize protein dynamics, and define ligand-binding mechanisms.
Structure · Function · Biomaterials
We investigate the structure, function, and design potential of bacterial lipoproteins.
Our approach
From basic science to applied research
Our research begins with fundamental questions about protein structure, function, and molecular recognition. We then apply these discoveries to engineer new biomaterials and develop technologies inspired by bacterial biology.
Explore our workFunction
Connect protein structure and ligand recognition to bacterial function and physiology.
Possibility
Translate mechanistic insight into new tools and therapeutic directions.
Publications
Explore our discoveries.
Our work spans bacterial lipoproteins, protein structure and dynamics, biomaterials, and molecular mechanisms.
LipoLoad:RB: a nanocarrier comprising bacterial lipoprotein LipoMetQ and Rose Bengal
Qianqiao Liu, Marc A. Arslanian, Matthew A. Treviño, Nirk E. Quispe Calla, Francesca Starvaggi, Naima G. Sharaf
Nature ArticleDevelopment and characterization of LipoCatch, a lipoprotein-based biomaterial
Francesca Starvaggi, Claire J. Stewart, Marc Arslanian, Matthew Treviño, Naima Sharaf
RSC ArticleStructural basis for selective thymidine binding by the Borrelia burgdorferi substrate-binding protein BmpA
Qianqiao Liu, Victor A. Nunez, Daniel Fernandez, Claire J. Stewart, Naima G. Sharaf
PubMedExpression, purification, and characterization of diacylated Lipo-YcjN from Escherichia coli
Matthew A. Trevino, Kofi A. Amankwah, Daniel Fernandez, Scott A. Weston, Claire J. Stewart, Jaime Morales Gallardo, Mona Shahgholi, Naima G. Sharaf
PubMedCorrection to: NMR structure of the HIV-1 reverse transcriptase thumb subdomain
Naima G. Sharaf, Andrew E. Brereton, In-Ja L. Byeon, P. Andrew Karplus, Angela M. Gronenborn
PubMedThe HIV-1 p66 homodimeric RT exhibits different conformations in the binding-competent and -incompetent NNRTI site
Naima G. Sharaf, Zhaoyong Xi, Rieko Ishima, Angela M. Gronenborn
PubMedNMR structure of the HIV-1 reverse transcriptase thumb subdomain
Naima G. Sharaf, Andrew E. Brereton, In-Ja L. Byeon, P. Andrew Karplus, Angela M. Gronenborn
PubMedConformational Plasticity of the NNRTI-Binding Pocket in HIV-1 Reverse Transcriptase: A Fluorine Nuclear Magnetic Resonance Study
Naima G. Sharaf, Rieko Ishima, Angela M. Gronenborn
PubMed(19)F-modified proteins and (19)F-containing ligands as tools in solution NMR studies of protein interactions
Naima G. Sharaf, Angela M. Gronenborn
PubMedThe p66 immature precursor of HIV-1 reverse transcriptase
Naima G. Sharaf, Eric Poliner, Ryan L. Slack, Martin T. Christen, In-Ja L. Byeon, Michael A. Parniak, Angela M. Gronenborn, Rieko Ishima
PubMedA bioreactor for in-cell protein NMR
Naima G. Sharaf, Christopher O. Barnes, Lisa M. Charlton, Gregory B. Young, Gary J. Pielak
PubMed